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vector red chromogen substrate  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vector red chromogen substrate
    Vector Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+chromogenic+substrate/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc12628778-299-51-55
    Average 96 stars, based on 1934 article reviews
    vector red chromogen substrate - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Compositions and methods for the treatment of aberrant angiogenesis
    Article Snippet: .. Immunohistological Staining: FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase (HRP)-labeled avidin (PerkinElmer), tyramide signal amplification (TSA, PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories) and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; Dako) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described (Kim L A, et al. (2015) Mol Vis 21:673-687).

    Article Title: miR-548j-5p regulates angiogenesis in peripheral artery disease
    Article Snippet: The ischemic muscles were embedded in paraffin, and then deparaffinized, followed by incubation with rat monoclonal antibodies against murine CD31 (BD PharMingen, San Diego, CA, USA). .. New capillaries were identified based on morphology and positive staining for CD31 using the avidin–biotin complex technique and Vector Red Chromogenic substrate (Vector Laboratories, Burlingame, CA, USA) after counterstaining with hematoxylin. ..

    Article Title: miR-548j-5p Regulates Angiogenesis in Peripheral Artery Disease
    Article Snippet: The ischemic muscles were embedded in para n, and then depara nized to incubate them with rat monoclonal antibodies against murine CD31 (BD PharMingen, San Diego, CA, USA). .. New capillaries were identi ed based on morphology and positive staining for CD31 using the avidin-biotin-complex technique and Vector Red Chromogenic substrate (Vector Laboratories, Burlingame, CA, USA) after counterstaining with hematoxylin. ..

    Incubation:

    Article Title: Compositions and methods for the treatment of aberrant angiogenesis
    Article Snippet: .. Immunohistological Staining: FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase (HRP)-labeled avidin (PerkinElmer), tyramide signal amplification (TSA, PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories) and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; Dako) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described (Kim L A, et al. (2015) Mol Vis 21:673-687).

    Article Title: Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis
    Article Snippet: Small Interfering RNA Gene Knockdown Small interfering RNA (siRNA) (75 nmol/L) (Integrated DNA Technologies; sequences in Supplementary Table 2 ) were transfected for 6–8 h using DharmaFECT 1 (GE Life Sciences/Dharmacon) in Opti-MEM (Life Technologies). .. Immunostaining FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz Biotechnology) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase–labeled avidin (PerkinElmer), tyramide signal amplification (PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories), and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; DakoCytomation) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described ( 8 ).

    Avidin-Biotin Assay:

    Article Title: Compositions and methods for the treatment of aberrant angiogenesis
    Article Snippet: .. Immunohistological Staining: FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase (HRP)-labeled avidin (PerkinElmer), tyramide signal amplification (TSA, PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories) and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; Dako) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described (Kim L A, et al. (2015) Mol Vis 21:673-687).

    Article Title: Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis
    Article Snippet: Small Interfering RNA Gene Knockdown Small interfering RNA (siRNA) (75 nmol/L) (Integrated DNA Technologies; sequences in Supplementary Table 2 ) were transfected for 6–8 h using DharmaFECT 1 (GE Life Sciences/Dharmacon) in Opti-MEM (Life Technologies). .. Immunostaining FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz Biotechnology) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase–labeled avidin (PerkinElmer), tyramide signal amplification (PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories), and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; DakoCytomation) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described ( 8 ).

    Article Title: miR-548j-5p regulates angiogenesis in peripheral artery disease
    Article Snippet: The ischemic muscles were embedded in paraffin, and then deparaffinized, followed by incubation with rat monoclonal antibodies against murine CD31 (BD PharMingen, San Diego, CA, USA). .. New capillaries were identified based on morphology and positive staining for CD31 using the avidin–biotin complex technique and Vector Red Chromogenic substrate (Vector Laboratories, Burlingame, CA, USA) after counterstaining with hematoxylin. ..


    Amplification:

    Article Title: Compositions and methods for the treatment of aberrant angiogenesis
    Article Snippet: .. Immunohistological Staining: FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase (HRP)-labeled avidin (PerkinElmer), tyramide signal amplification (TSA, PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories) and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; Dako) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described (Kim L A, et al. (2015) Mol Vis 21:673-687).

    Article Title: Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis
    Article Snippet: Small Interfering RNA Gene Knockdown Small interfering RNA (siRNA) (75 nmol/L) (Integrated DNA Technologies; sequences in Supplementary Table 2 ) were transfected for 6–8 h using DharmaFECT 1 (GE Life Sciences/Dharmacon) in Opti-MEM (Life Technologies). .. Immunostaining FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz Biotechnology) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase–labeled avidin (PerkinElmer), tyramide signal amplification (PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories), and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; DakoCytomation) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described ( 8 ).

    Immunostaining:

    Article Title: Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis
    Article Snippet: Small Interfering RNA Gene Knockdown Small interfering RNA (siRNA) (75 nmol/L) (Integrated DNA Technologies; sequences in Supplementary Table 2 ) were transfected for 6–8 h using DharmaFECT 1 (GE Life Sciences/Dharmacon) in Opti-MEM (Life Technologies). .. Immunostaining FVMs and cells were incubated with mouse anti-RUNX1 (Santa Cruz Biotechnology) followed by biotinylated secondary antibody (Vector Laboratories), horseradish peroxidase–labeled avidin (PerkinElmer), tyramide signal amplification (PerkinElmer), Vector Red chromogenic substrate (Vector Laboratories), and Gill no. 3 hematoxylin counterstain (Sigma-Aldrich). .. Immunofluorescent staining for CD31 (mouse anti-CD31; DakoCytomation) and Ki67 (rabbit anti-Ki67; Novus Biologicals) was performed as previously described ( 8 ).

    other:

    Article Title: Increased burden of mitochondrial DNA deletions and point mutations in early-onset age-related hearing loss in mitochondrial mutator mice
    Article Snippet: Sections were rinsed in tap water and nuclei were counterstained in Vector methyl green (Vector Laboratories, H-3402) at 60 °C for 3 min.



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    Vector Laboratories chromogenic alkaline phosphatase solution
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Vector Laboratories alkaline phosphatase chromogen substrate
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Vector Laboratories vector red substrate chromogen solution
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection